The Construction of the Transient Expression Vector for the Foreign Green Fluorescent Protein (GFP) and the Study of Its Protein Expression

Abstract

Transient gene expression facilitates the study and analysis in gene function and its short-term effect. GFP (Green Fluorescent Protein) has been studying for years as a marker of gene expression and protein targeting. Thus, establishing a transient expression vector for GFP is conducive to gene function research, cell process analysis, and drug screening. Through plasmid DNA isolation and purification, PCR amplification of GFP, and plasmid recombination, the vector is established.