Progress in the Application of Enzyme Improvement Design Technology

Abstract

At present, enzyme improvement is becoming more and more indispensable in production and scientific research, but there is no accurate improvement method recommendation for enzymes of different types and structures. In the current research, the author has analyzed several enzyme improvement methods and given suggestions for improvement methods for different types of enzymes. However, with the development of biotechnology, enzyme improvement technology is also constantly updated and iterated. This article analyzes the most commonly used mainstream methods. This article compares and studies three methods of enzyme improvement design, namely rational design, semi-rational design and random design, and conducts a detailed study and analysis of these three improvement methods, and obtains the most suitable improvement methods for different types of enzymes. It can provide a reference for the selection of enzyme improvement methods in future research, but the screening of methods when the enzyme meets the conditions of multiple improvement methods at the same time has not been solved. Future research can focus on the screening and prediction of improvement methods.