Single - cell Sequencing in Stem Cell Nanotargeted Therapy for Triple - negative Breast Cancer

Abstract

Triple-negative breast cancer (TNBC) lacks the expression of three Targeted hormone receptors, namely Estrogen receptor (ER), Progesterone receptor (PR), and Human epidermal growth factor receptor 2 (HER2), leading to difficulties in implementing Targeted therapy. Its high Heterogeneity is primarily regulated by the Interaction between the Tumor microenvironment (TME) and Cancer stem cell subpopulations (CSCs). The Immunosuppressive microenvironment within the TME promotes the survival and Metastasis of tumor stem cells, while CSCs, by secreting Immune checkpoint molecules, in turn promote the immunosuppressive microenvironment and simultaneously remodel the TME.This study, by investigating their interactions and the integration of single-cell RNA sequencing (sc-RNAseq) technology, aims to identify the Heterogeneity of Triple-negative breast cancer stem cells (TNBC-CSCs) and their surface markers, to elucidate the molecular characteristics, regulatory mechanisms, and functional properties of CSCs, and to seek prognosis-related therapeutic targets for different subtypes of TNBC. Simultaneously, novel nanomaterials, such as nCOF, will be utilized to deliver drugs to overcome the difficulties in drug delivery for TNBC, thereby achieving precise drug release.